84-2300-27　［取扱停止］ATP2A2 (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic Reticulum Type, slow twitch skeletal muscle Isoform, Endoplasmic Reticulum class 1/2 Ca(2+) ATPase, ATP2B) 100ul　220938

特徴

• ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations.
• The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases.
• The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle.
• The SERCAs mediate Ca2+ uptake into intracellular stores.
• SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation.
• The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle.
• The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types.
• The SERCA3 gene is not as well characterized and is found in non-muscle cells.
• SERCA2 plays an important part in regulating cardiac contractile function.
• SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells.
• SERCA1, SERCA2 and SERCA3 all undergo alternative splicing.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.