This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen.
Isoform 2 is involved in the regulation of the contraction/relaxation cycle.Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta.
Also expressed at lower levels in heart and skeletal muscle.
Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle.
Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma.
Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta.
Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ATP2A2. Species Crossreactivity: Human, mouse, rat
