84-2299-93　［取扱停止］ATG3 (Apg 3, APG3, APG3 Autophagy 3 like, APG3 like, APG3, S. cerevisiae, Homolog of, APG3-like, APG3L, Apg3p, ATG 3, ATG3, ATG3 Autophagy Related 3 Homolog, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), ATG3_HUMAN, Autophagy 3, S. cerevisiae, Homolog of, Autophagy Apg3p/Aut1p like, Autophagy Related 3, Autophagy Related Protein 3, Autophagy-Related Protein 3, ...　220920

特徴

• Atg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 orPC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressedin a variety of tissues with predominant levels found in kidney, placenta, liver,heart and skeletal muscle.
• Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins).
• The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease.
• The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE.
• This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.