ATG13 an autophagy factor required for autophagosome formation.
Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.
Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
Phosphorylated by ULK1 and ULK2.
Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin.
Part of a complex consisting of ATG13, ULK1 and RB1CC1.
Interacts with ATG101.
Interacts with ULK1 and -2 via C-terminus.
Three isoforms of the human protein are produced by alternative splicing.
Note: This description may include information from UniProtKB.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ATG13. Species Crossreactivity: Human, mouse, rat
