Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements.
The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA.
There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3.
A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins, with highest homology to ATF-4.
ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase.
ATF-7 homodimers bind specifically to CRE elements.
ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation.
ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1.
In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ATF-7. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 40-90 of Human ATF-7.
