Ataxin-2, the gene product of the human spinocerebellar ataxia type 2 (SCA2/ATXN2) gene, is a basic protein with two domains (Sm1 and Sm2) implicated in RNA splicing and protein interaction.
Ataxin-2 interacts with a putative RNA-binding protein ataxin-2-binding-protein 1 (A2BP1), which is expressed in muscle and brain.
Ataxin-2 is ubiquitously expressed with highest levels in the cytoplasm of Purkinje cells.
Both A2BP1 and ataxin-2 are localized to the trans-Golgi network.
Mice expressing ataxin-2 with polyglutamine show progressive functional deficits accompanied by loss of Purkinje cell dendritic arbor and eventually loss of Purkinje cells.
In conclusion, expansion of ataxin-2 results in spinocerebellar ataxia type 2, which affects the cerebellum and other areas of the central nervous system.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Ataxin-2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 740-788 of human Ataxin-2.
