Acid sphingomyelinase (ASM) is a lysosomal protein that hydrolyzes sphingomyelin to ceramide and phosphocholine.
The ASM gene encodes three proteins, ASM-1, ASM-2 and ASM-3, of which ASM-1 is the only ASM gene product that is a catalytically active enzyme.
Deficiency of ASM is associated with type A and type B Niemann-Pick disease.
Type A is a fatal neurodegenerative disorder seen in infancy and resulting in death by age three, whereas type B is a non-neuropathic disease that has a later onset.
During monocytic cell differentiation, the expression of ASM is upregulated by the combined actions of AP-2 and Sp1 transcription factors.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human ASM. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in human ASM. Swiss-Prot: P17405
