Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors.
The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38.
The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5).
MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway.
Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3.
MEK kinase-4 has been shown to specifically activate the JNK pathway.
ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ASK1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 43-90 of Human ASK1.
