Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors.
ARHGEF5 (Rho guanine nucleotide exchange factor (GEF) 5), also known as P60 or transforming immortalized mammary oncogene (TIM), is a 1597 amino acid cytoplasmic protein.
ARHGEF5 is believed to form a complex with G proteins and be involved in the initiation of Rho-dependent signals.
It has also been suggested that ARHGEF5 may be involved in the organization of the cytoskeleton.
ARHGEF5 contains one PH domain, one DH domain and one SH3 domain.
Existing as two isoforms produced by alternative splicing, ARHGEF5 is mainly expressed in pancreas, liver, kidney, placenta and lung.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ARHGEF5. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1310-1355 of Human ARHGEF5.
