The INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF that restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively.
It is among the most frequently mutated tumor suppressor loci in human cancer.
Both p16INK4a and p19ARF act as cell proliferation inhibitors.
The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation.
In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein.
p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways.
Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process.
Expression of p19ARF but not p16INK4a induces apoptosis in Ab-MLV-transformed pre-B cells.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of p14 ARF. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 65-121 of Human p19 ARF.
