This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg).
Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH.
The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice.
The other adrenergic receptor, alpha1D-AR, were not changed by the treatment.
Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment.
These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of AR-alpha2A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 331-380 of Human AR-α2A.
