Apolipoproteins are protein components of plasma lipoproteins.
The apolipoprotein L gene family encodes six highly homologous proteins designated apoL 1-6, which are associated with large high density type lipoproteins (HDL).
The human apoL family maps to chromosome 22q12.3 within a 127,000-bp region.
ApoL2 and apoL3 may allow the binding of lipids to organ-elles or may be involved in the movement of lipids in the cytoplasm.
ApoL2 localizes to the cytoplasm and is widely expressed, with the highest levels observed in the lung, thymus, pancreas, placenta, adult brain and prostate.
The apoL2 protein is also detected in the spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver.
ApoL3 also localizes to the cytoplasm and is widely expressed, with highest levels detected in the prostate, lung and placenta.
It is also detected in kidney, bone marrow, spleen, thymus, spinal cord, adrenal gland, salivary gland, trachea and mammary gland.
Lower levels of apoL3 are observed in the brain, heart, fetal liver, pancreas and testis.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity:Recognizes endogenous levels of ApoL2. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:IF IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to amino acids 196-240of Human ApoL2.