Apolipoprotein-E (ApoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles.
It can serve as a ligand for several lipoprotein receptors, including the LDL (apoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor.
apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20% of VLDL (very low density lipoprotein) and 1-2% of HDL (high density lipoprotein).
Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by one to two amino acids.
Estrogen receptor has been shown to upregulate apoE gene expression via the ERamediated pathway, indicating a potential role for apoE in atherosclerosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ApoE. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 264-309 of Human ApoE.
