The apelin receptor (APLNR) is a G protein-coupled integral membrane protein exhibiting a hypothalamic distribution in brain, glial cells, astrocytes and neuronal subpopulations.
APLNR is bound by its cognate neuropeptide ligand, apelin, promoting receptor internalization to the nucleus and dose-dependent inhibition of forskolin-induced cAMP production.
However, deletion studies of the apelin agonist have shown that internalization is not mandatory for decreasing vasopressin release, a hypotensive action of APLNR signaling.
Further evidence for functional dissociation of APLNR stimulation and internalization was exhibited in vitro using mutational studies of a nuclear localization signal sequence.
These findings may suggest the presence of multiple, functionally-differing conformational states for the receptor.
Stress studies in rodents have shown APLNR is under negative regulation by glucocorticoids and may be involved in controlling hypothalamic function.
APLNR also functions as an alternate coreceptor with CD4 for HIV-1 infection.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of APLNR. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 150-200 of Human APLNR.
