84-2297-01　［取扱停止］APEX1 (AP Lyase, APE 1, APE, APE-1, APEN, APEX 1, APEX, APEX nuclease (multifunctional DNA Repair Enzyme) 1, Apex nuclease 1, Apex nuclease, APEX1, Apurinic Endonuclease, Apurinic-apyrimidinic Endonuclease 1, Apurinic/apyrimidinic (abasic) Endonuclease, Apurinic/apyrimidinic Endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic ...　220774

特徴

• The role of transcription factors in the regulation of gene expression is well established.
• Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status.
• Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun.
• Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun.
• A similar mode of regulation has been recently proposed for other nuclear transcription factors.
• Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1).
• Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.