APEH (Acyl-peptide hydrolase), also known as APH, OPH or ACPH, is a 732 amino acid cytoplasmic protein that exists as a homotetramer and functions to catalyze the hydrolysis of N-terminal acetylated amino acids from small acetylated peptides.
Once hydrolyzed from the target peptide, the acetyl amino acid is further processed by an aminoacylase to produce acetate and a free amino acid.
The gene encoding human APEH maps to a region on chromosome 3 that is deleted in various types of cancers, including renal cell carcinoma and small cell lung carcinoma, suggesting that APEH may be involved in tumor transformation events.
Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of APEH. Species Crossreactivity: Human, mouse, rat
