84-2296-80　［取扱停止］AP2M1 (Adapter-Related Protein Complex 2 mu Subunit, Adaptin mu 1, Adaptin-mu2, Adaptor Protein Complex AP 2 Subunit mu, Adaptor Protein Complex AP-2 Subunit mu, Adaptor Related Protein Complex 2 mu 1 Subunit, AP 2 mu 2 Chain, AP-2 Complex Subunit mu, AP-2 mu Chain, AP2M 1, Ap2m1, AP2M1_HUMAN, AP50, CLAPM1, Clathrin adaptor Complex AP2 mu Subunit, Clathrin assembl ...　220764

特徴

• The AP-2 coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3).
• Each AP-2 heterotetramer is composed of alpha, beta, μ, and sigma protein subunits.
• The 50kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates interaction between the cargo protein and the clathrin-coated pit (1-4).
• The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif yXXφ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit.
• Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6).
• AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10), and ion channel proteins (11).
• Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13).
• Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.