Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases.
The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region.
Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2.
Preliminary biochemical analyses of Ang-1 reveal a potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus.
Ang-1 is an angiogenic factor that is thought to be involved in endothelial development.
A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ANGPT2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human ANGPT2.
