84-2295-91　［取扱停止］AMPK alpha1, phosphorylated (S486) (5'-AMP-Activated Protein Kinase Catalytic Subunit alpha-1, AMPK Subunit alpha-1, Acetyl-CoA Carboxylase Kinase, ACACA Kinase, Hydroxymethylglutaryl-CoA Reductase Kinase, HMGCR Kinase, au-Protein Kinase PRKAA1, PRKAA1, AMPK1) 50ul　220719

特徴

• AMPK is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory beta and y subunits.
• It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
• AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.
• Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
• The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively.
• Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein.
• The human AMPKy1 gene encodes a 331 amino acid protein.
• Human AMPKy2 and AMPKy3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.