ALy (also designated THO complex subunit 4, THOC4, REF1, Refbp1 and BEF) is the mammalian homolog of the yeast mRNA export factor yralp.
A transcriptional coactivator, ALy belongs to the cytidylyltransferase family and is important in mRNA processing and export.
During spliceosome assemby, it is recruited to messenger ribonucleoprotein (mRNP) complexes and becomes tightly associated with the spliced mRNP.
Consistent with splicing-dependent recruitment, ALy colocalizes with splicing factors in the nucleus.
It promotes transcriptional activation by promoting the dimerization of transcription factors containing basic leucine zipper domains.
Although ubiquitously expressed, ALy specifically associates with the activation domains of LEF-1 and AML-1, both of which are protein components of the TCR alpha enhancer complex.
Research indicates that ALy may mediate context-dependent transcriptional activation by facilitating the functional collaboration of multiple proteins in the TCR alpha enhancer complex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ALY. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 130-182 of Human ALY.
