ALKBH3 (alkB, alkylation repair homolog 3), also known as ABH3, PCA1 (prostate cancer antigen 1), DEPC1 or DEPC-1, is a 286 amino acid member of the AlkB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates.
ALKBH3 is one of many homologs of the Escherichia coli protein, AlkB.
ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus.
It associates with iron and 2-oxoglutarate, coupling the oxidation of substrates to the conversion of 2-oxoglutarate into succinate and CO2.
Via oxidative demethylation, ALKBH3 repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA.
Its activity is stimulated by ascorbate.
Two isoforms exist for ALKBH3 due to alternative splicing of the gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ALKBH3. Species Crossreactivity: Human, mouse, rat
