Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate.
Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase C.
Aldolase A, B and C arose from a common ancestral gene from which Aldolase B first diverged.
Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years.
Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease.
Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells.
Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase Cnegative Purkinje cells.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Aldolase A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Aldolase A.
