AKT, also known as protein kinase B (PKB), is a 57kDa serine/threonine protein kinase.
There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being ~82% identical with the AKT1 isoform.
Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain.
AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways.
Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of AKT, phosphorylated T450. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 81.
Shelf Life：1year
Swiss Prot Number：P31749/P31751/Q9Y243
EU Commodity Code：30021010
商品名：AKT, phosphorylated (T450) (RAC-alpha Serine/threonine-Protein Kinase, Protein Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-Oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC, PKBalpha, RAC-beta Serine/threonine-Protein Kinase, Protein Kinase Akt-2, Protein Kinase B beta, PKB beta, RAC-PK-beta, AKT2, PKBb, RAC-gamma Serine/threonine-Protein Kinase, Protein Kinase Akt-3, Protein Kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, AKT3, PKBG, PKBy) 50ul
