The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates.
Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell.
To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins.
The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP).
AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle.
During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression.
Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit.
In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of AKAP8. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 373-418 of Human AKAP8.
