84-2294-56　［取扱停止］AKAP 5 (A Kinase (PRKA) anchor Protein 5, A Kinase anchor Protein 5, A Kinase anchor Protein 79kDa, A-Kinase anchor Protein 5, A-Kinase anchor Protein 79kD, AKAP 5, AKAP 79, AKAP-5, AKAP5, AKAP75, AKAP79) 50ul　220647

特徴

• AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell.
• Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RIalpha, RIIalpha, RIbeta, or RIIbeta), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location.
• AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities.
• AKAP5 specifically binds to the regulatory subunit of PKAIIbeta, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions.
• The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.