The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates.
Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell.
To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins.
The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP).
AKAP 13, also known as Brx (breast cancer nuclear receptor-binding auxiliary protein), Lbc (lymphoid blast crisis oncogene), HA-3 or Ht31 (human thyroid-anchoring protein 31), functions as a cAMP-dependent scaffold anchor for PKA and also has Rho-GEF activity.
It is known to regulate TLR2 signaling, NFκB activation, protein kinase D activation and participate in Actin stress fiber formation.
Seven isoforms exist for AKAP 13 and, depending on the isoform, it localizes to the cytoplasm, nucleus or cell membrane.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of AKAP 13. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 700-750 of Human AKAP 13.
