Interferon-inducible protein AIM2 (absent in melanoma 2) is a 343 amino acid protein belonging to the HIN-200 family.
Induced by IFN-y, AIM2 is thought to act as a tumor suppressor by repressing NFκB transcriptional activity.
Localized to the nucleus, AIM2 contains one DAPIN domain and one HIN-200 domain.
The DAPIN domain is composed mostly of alpha-helixes and is a protein-protein interaction domain capable of binding other DAPIN domains.
The HIN-200 domain has been shown to bind directly to DNA, which, along with the binding of another protein ASC, results in the activation of caspase-1.
AIM2 is present as a homodimer and is expressed highly in small intestine, testis, peripheral blood leukocytes and spleen.
Defects in AIM2 are believed to be a cause of microsatellite unstable colon cancers.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of AIM2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 51-100 of Human AIM2.
