Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma).
Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure.
Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Alpha and gamma adducins are ubiquitously expressed.
In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues.
Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C.
Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Adducin alpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 700-750 of Human Adducin α.
