Tumor necrosis factor beta (TNFbeta), also known as lymphotoxin, is a pleiotropic cytokine.
TNFalpha, also known as cachetin, is a cytokine that binds to the same receptors, producing an array of effects similar to those of TNFbeta.
TNFbeta and TNFalpha share 30% amino acid homology and have similar biological activities.
TNFbeta is produced by activated lymphocytes, including CD4+ T helper cell type 1 lymphocytes, CD8+ lymphocytes and certain B lymphoblastoid cell lines.
TNFalpha is produced by several different cell types, including lymphocytes, neutrophils and macrophages.
TNFbeta and TNFalpha can modulate many immune and inflammatory functions while having the ability to inhibit tumor growth.
TACE (for TNFalpha converting enzyme) is a metalloproteinase that cleaves the membrane-bound TNFalpha precursor to release soluble TNFalpha.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Cleaved-ADAM17. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 180-230 of Human Cleaved-ADAM17.
