Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY-1, is a member of the largest metallopeptidase family called M20A.
Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen.
It is the most abundant of the aminoacylases.
Aminoacylase-1 localizes to the cytoplasm and catalyzes the hydrolysis of N-acylated or N-acetylated amino acids.
In addition, Aminoacylase-1 is a sphingosine kinase 1 (SphK1)-interacting protein and may also play a role in regulating responses of the cell to oxidative stress.
The gene encoding Aminoacylase-1 is evolutionarily conserved in fish, frog, mouse, rat and human.
Deficiency in the Aminoacylase-1 protein may result in defects of brain metabolism and function.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ACY1. Species Crossreactivity: Human
