All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein.
Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells.
While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes.
At least six types of actin are present in mammalian tissues and fall into three classes.
-actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton.
Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:3000-1:10000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of beta-Actin. Species Crossreactivity: Human, Mouse, Rat
