Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes.
It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer.
Adiponectin levels are decreased in obese and insulin-resistant mice and humans, suggesting that this adipokine is critical to maintain insulin sensitivity.
Adiponectin stimulates the phosphorylation of AMPKalpha at Thr172 and activates AMPK in skeletal muscle.
It also stimulates glucose uptake in myocytes.
The block of AMPK activation by a dominant-negative AMPKalpha2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK.
Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway.
Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes, indicating the importance of multimerization for adiponectin activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Acrp30. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 22-76 of Human Acrp30.
