Regulation of intracellular concentrations of flavoenzymes and flavin coenzymes is essential for proper cell homeostasis.
Red cell acid phosphatase, known as ACP1, catalyzes the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol.
ACP is a genetically polymorphic, cytoplasmic low-molecular-weight flavin mononucleotide phosphatase that regulates the intracellular concentrations of flavin coenzymes.
The human ACP1 gene maps to chromosome 2p25 and encodes a pair of isozymes, Bf (alpha) and Bs (beta).
The ACP1 alpha and beta isozymes are not glycosylated.
Both ACP1-alpha and ACP1-beta isozymes are 157 amino acids in length; however the two forms differ in sequence over an internal 34 residue segment.
The two isoforms are believed to differ in substrate specificity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of ACP1. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Recombinant full length Human ACP1.
Shelf Life:1year
Swiss Prot Number:P24666
EU Commodity Code:30021010
商品名:ACP1 (Acid Phosphatase 1 Soluble, Acid Phosphatase of Erythrocyte, ACP1, Adipocyte acid Phosphatase, Cytoplasmic phosphotyrosyl Protein Phosphatase, HAAP, LMW-PTP, LMW-PTPase, Low Molecular weight cytosolic acid Phosphatase, Low Molecular weight phosphoTyrosine Protein Phosphatase, PAP1, PAP2, PPAC_HUMAN, Protein Tyrosine Phosphatase, PTPase, Purple acid Phosphatase, Red Cell acid Phosphatase 1) 50ul