ACOT8 (Acyl-CoA thioesterase 8) also designated, TEII p35 or hTE, is a novel human thioesterase that has been found to interact with the HIV protein Nef using yeast two-hybrid screening.
Nef is an auxiliary gene of the human immunodeficiency virus (HIV) which facilitates virus replication and enhances infectivity.
The roles of Nef in HIV-infected cells are likely to be mediated by specific interactions with cellular proteins.
The interaction between Nef and ACOT8 is correlated with CD4 downregulation, suggesting that ACOT8 may be involved in Nef-mediated CD4 downregulation in HIV-infected cells.
ACOT8 is 42% identical to thioesterase II from Escherichia coli, and it has no significant homology with the two types of animal thioesterases that have previously been cloned (type I and type II thioesterases).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ACOT8. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 151-200 of Human ACOT8.
