The Ras-related Rho subfamily of GTP-binding proteins (p21s), which includes Rho, Rac and Cdc42Hs, is implicated in different aspects of cytoskeletal organization.
These proteins resemble Ras p21 in that their active GTP-bound form is inactivated by intrinsic hydrolysis of the GTP to GDP, which can be stimulated by GTPase-activating proteins (GAPs).
ACK, a tyrosine kinase that specifically binds Cdc42Hs in its GTP-bound form, has been described.
This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain and inhibits both the intrinsic and GAP-stimulated GTPase activity of Cdc42Hs.
These findings suggest that ACK may represent a new class of proteins that sustains the GTP-bound active form of the Rho subfamily of GTP binding proteins and which is directly linked to a tyrosine phosphorylation pathway.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ACK. Species Crossreactivity: Human, mouse, rat
