Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions.Alternative mRNA splicing gives rise to three forms of AChE.
The T form, also known as the asymmetric form, is soluble and is present in synapses.
The H form is also known as the globular form and is present on the outer surfaces of cell membranes.
The R form is not known to be a functional species.
AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail.
The catalytic subunits of AChE are oligomers composed of disulfide-linked homodimers.
The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease.
However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of AChE. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 560-600 of Human AChE.
