Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis.
Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle.
ACCalpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCbeta (ACC2) may control mitochondrial fatty acid oxidation.
These two isoforms of ACC control the amount of fatty acids in the cells.
The catalytic function of ACCalpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme.
The gene encoding ACCalpha, which maps to human chromosome 17, encodes the 265kDa alpha form of ACC, which is the major ACC in lipogenic tissues.
The catalytic core of ACCbeta is homologous to that of ACCalpha except for an additional peptide of about 150 amino acids at the N-terminus.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ACCalpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-100 of Human ACCα.
