The Abl interactor proteins, Abi-1 and Abi-2, are SH3-domain containing proteins that bind to the proline-rich motifs of Abl and activate the kinase function.
Two splice variants of Abi-1 are widely expressed, with the highest levels found in bone marrow,spleen, brain and testis.
Abi-1 and Abi-2 are thought to negatively regulate cell growth and transformation, including cellular transformation through v-Abl.
ABI1, the gene encoding Abi-1, has been shown to translocate and fuse with MLL (mixed lineage leukemia) gene in some cases of acute myeloid leukemia (AML).
The Abi proteins have also been identified as mediators of cell motility by regulating Actin polymerization in lamellipodia and filopodia.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Abi-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 170-220 of Human Abi-1.
