ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family.
ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis.
There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White.
ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4.
ABCC4 is widely expressed in cells and tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines.
ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds .
The diversity of substrates determines the biological functions of ABCC4.
It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells.
ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostagladins.
Researchers have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy.
ABCC4 localizes to both plasma membrane and intracellular membranous structures.
Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki desease, a genetic childhood disease characterized by vasculitis .
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ABCC4. Species Crossreactivity: Human, mouse, rat
