84-2291-40　［取扱停止］AATF (Protein AATF, Apoptosis-antagonizing Transcription Factor, Rb-Binding Protein Che-1, AATF, CHE1, DED, HSPC277) 100ul　220489

特徴

• Che-1, also known as apoptosis-antagonizing transcription factor (AATF), is a widely expressed nuclear protein that belongs to the AATF family.
• Hyperphosphorylated during the G1/S phase transition, Che-1 may function as a general inhibitor of the histone deacetylase HDAC1.
• Che-1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression.
• Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che-1.
• It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer’s disease (AD), also known as presenile and senile dementia.
• PAR4 is a leucine zipper protein that is pro-apoptotic and associated with neuronal degeneration in AD.
• Che-1 interaction with PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity.
• Che-1 also colocalizes with PAR4 in both cytoplasmic and nuclear compartments, and interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.