Classically, adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins.
The binding of an agonist to a Gs-coupled receptor (i.e., alpha beta-adrenergic receptor) catalyzes the exchange of GDP (bound to Galpha s) for GTP, dissociation of GTP-Galpha s from Gbg and Galpha s-mediated activation of adenylyl cyclase.
The most abundant cerebral adenylyl cyclases appears to be adenylyl cyclase IX.
AC IX is confined to the gray matter and its expression is mainly neuronal, with its highest expression located at the hippocampus.
ACIX is also expressed in heart, pancreas and thyrocytes.
AC I and AC IX are regulated reciprocally by intracellular free Ca2+.
The inhibition of AC IX by Ca2+ is blocked by the calcineurin inhibitors FK506 and cyclosporin A.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of A Cyclase. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 971-1020 of Human A Cyclase IX.
