84-2291-19　［取扱停止］53BP1 (Tumor Suppressor p53-Binding Protein 1, 53 BP1, p53-Binding Protein 1, p53BP1, TP53BP1, p53-BP1) 50ul　220479

特徴

• The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53.
• The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors.
• Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation.
• Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression.
• 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus.
• The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus.
• 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.