84-2290-91　［取扱停止］4E-BP1 (Eukaryotic Translation Initiation Factor 4E-Binding Protein 1, 4E-BP1, eIF4E-Binding Protein 1, Phosphorylated Heat- and acid-stable Protein Regulated by insulin 1, PHAS-I, EIF4EBP1, 4EBP1, PHAS I, PHASI) 100ul　220465

特徴

• The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure.
• eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex.
• The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function.
• Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation.
• A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.