84-2289-89　Antithrombin III (ATIII) (PE) 100ul　A2298-22-PE

特徴

• Antithrombin III (ATIII) is a plasma protein synthesized in the liver.
• Normal plasma levels are 115-160mg/L.
• ATIII consists of a single polypeptide chain and has a molecular weight of 58kD.
• ATIII is a serine protease inhibitor and through this action regulates blood coagulation.
• ATIII also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold.
• Applications:Suitable for use in Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis.
• Other applications have not been tested.
• Recommended Dilutions:RID: 6ul antiserum/cm2 gel vs.
• 5ul neat - 1:10 dilution human plasma.
• Double Diffusion: 10ul antiserum vs.
• 5ul plasma IEP: 100ul antiserum vs.
• 5ul plasma gives single arc
• Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.