Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome.
The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death.
Their molecular masses range between 27 and 30kD.
At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E.
Their aa sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop.
Enterotoxins are extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of these substances.
Enterotoxins are Superantigens, inducing polyclonal T cell activation by binding to the TCR and to the alpha chain of the MHC II molecule simultaneously.
Applications:Suitable for use in double diffusion.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：S.aureus
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Staphylococcus aureus Enterotoxin B. Does not react with other Staphylococcus toxins.
