84-2289-75　［取扱停止］Protein A (HRP) 100ul　P9102-14J-HRP

特徴

• Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
• Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
• Protein A may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site.
• These conjugates may be used to track immunoglobulins in histochemical, Western Blot and ELISA.
• Alternatively, Protein A may be immobilized onto a solid support to facilitate the purification and recovery of either polyclonal or monoclonal immunoglobulins.
• Immobilized protein A has recently been applied therapeutically to treat a variety of humoral diseases (Watson et al, 1989).
• Applications:Suitable for use in ELISA.
• Other applications not tested
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x ChI1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x ChI1906-14IgY, Chicken (AP) Rb x Ch
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.