Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver.
Plasma concentration of FIX is normally around 5ug/ml (87nM) in plasma.
The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease).
This is an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in FIX function.
In its proenzyme or zymogen form, FIX is a single chain molecule of 55kD.
It contains two EGF-like domains and an amino-terminal domain containing 12-g-carboxy-glutamic acid (Gla) residues.
These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions.
The activation of FIX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146-Ala147 and between Arg180-Val181.
The terminal activated product in either case is FIXab, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD.
FIX can also be cleaved into inactive products by thrombin and by elastase.
The activity of FIXab in plasma is inhibited by antithrombin.
This inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1: 50,000Western Blot: 1-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes mouse factor IX and mouse factor IX alpha.
