Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae-bacteria that inhabit the gastrointestinal tract.
Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Applications:Suitable for use in ELISA, Dot Blot and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes all E. coli species in immunogen, plus O157-H7. Recognizes more than 50 proteins by Western Blot.
Isotype：IgG
Calc Applications Abbrev：DB E WB
Calc Crossreactivity：Ec
Immunogen：Heat inactivated blend of 6 different strains of E. coli: TOP 10F, HB101, JM109, BL21, DH5 alpha and K12.
