84-2289-33　Antithrombin III (ATIII) (HRP) 100ul　A2298-22-HRP

特徴

• Antithrombin III (ATIII) is a plasma protein synthesized in the liver.
• Normal plasma levels are 115-160mg/L.
• ATIII consists of a single polypeptide chain and has a molecular weight of 58kD.
• ATIII is a serine protease inhibitor and through this action regulates blood coagulation.
• ATIII also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold.
• Applications:Suitable for use in Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis.
• Other applications have not been tested.
• Recommended Dilutions:RID: 6ul antiserum/cm2 gel vs.
• 5ul neat - 1:10 dilution human plasma.
• Double Diffusion: 10ul antiserum vs.
• 5ul plasma IEP: 100ul antiserum vs.
• 5ul plasma gives single arc
• Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.