Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae-bacteria that inhabit the gastrointestinal tract.
Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Applications:Suitable for use in FLISA, Dot Blot and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes all E. coli species in immunogen, plus O157-H7. Recognizes more than 50 proteins by Western Blot.
Isotype：IgG
Calc Applications Abbrev：DB FLISA WB
Calc Crossreactivity：Ec
Immunogen：Heat inactivated blend of 6 different strains of E. coli: TOP 10F, HB101, JM109, BL21, DH5 alpha and K12.
